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RAF 2 is a RuBis CO assembly factor in Arabidopsis thaliana
Author(s) -
Fristedt Rikard,
Hu Chen,
Wheatley Nicole,
Roy Laura M.,
Wachter Rebekka M.,
Savage Linda,
Harbinson Jeremy,
Kramer David M.,
Merchant Sabeeha S.,
Yeates Todd,
Croce Roberta
Publication year - 2018
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13849
Subject(s) - arabidopsis thaliana , mutant , chemistry , biology , biochemistry , gene
Summary Ribulose‐1,5‐bisphosphate carboxylase/oxygenase (RuBis CO ) catalyzes the reaction between gaseous carbon dioxide ( CO 2 ) and ribulose‐1,5‐bisphosphate. Although it is one of the most studied enzymes, the assembly mechanisms of the large hexadecameric RuBis CO is still emerging. In bacteria and in the C4 plant Zea mays , a protein with distant homology to p terin‐4α‐ c arbinolamine d ehydratase ( PCD ) has recently been shown to be involved in RuBis CO assembly. However, studies of the homologous PCD ‐like protein ( RAF 2, RuBis CO assembly factor 2) in the C3 plant Arabidopsis thaliana ( A . thaliana ) have so far focused on its role in hormone and stress signaling. We investigated whether A . thaliana RAF 2 is also involved in RuBis CO assembly. We localized RAF 2 to the soluble chloroplast stroma and demonstrated that raf2 A . thaliana mutant plants display a severe pale green phenotype with reduced levels of stromal RuBis CO . We concluded that the RAF 2 protein is probably involved in RuBis CO assembly in the C3 plant A . thaliana .