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Effect on transcriptome and metabolome of stacked transgenic maize containing insecticidal cry and glyphosate tolerance epsps genes
Author(s) -
Wang Xujing,
Zhang Xin,
Yang Jiangtao,
Wang Zhixing
Publication year - 2018
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13825
Subject(s) - metabolome , transcriptome , biology , gene , transformation (genetics) , transgene , genetically modified crops , genetics , microbiology and biotechnology , metabolomics , gene expression , bioinformatics
Summary Gene stacking is a developing trend in agricultural biotechnology. Unintended effects in stacked transgenic plants are safety issues considered by the public and researchers. Omics techniques provide useful tools to assess unintended effects. In this paper, stacked transgenic maize 12‐5× IE 034 that contained insecticidal cry and glyphosate tolerance G10‐epsps genes was obtained by crossing of transgenic maize varieties 12‐5 and IE 034. Transcriptome and metabolome analyses were performed for different maize varieties, including 12‐5× IE 034, 12‐5, IE 034, and conventional varieties collected from different provinces in China. The transcriptome results were as follows. The nine maize varieties had obvious differences in gene expression. There were 3561–5538 differentially expressed genes between 12‐5× IE 034 and its parents and transgenic receptor, which were far fewer than the number of differentially expressed genes in different traditional maize varieties. Cluster analysis indicated that there were close relationships between 12‐5× IE 034 and its parents. The metabolome results were as follows. For the nine detected maize varieties, the number of different metabolites ranged from 0 to 240. Compared with its parents, 12‐5 and IE 034, the hybrid variety 12‐5× IE 034 had 15 and 112 different metabolites, respectively. Hierarchical cluster analysis with Pearson's correlation analysis showed that the differences between 12‐5× IE 034 and its parents were fewer than those between other maize varieties. Shikimate pathway‐related genes and metabolites analysis results showed that the effects of hybrid stacking are less than those from transformation and differing genotypes. Thus, the differences due to breeding stack were fewer than those due to natural variation among maize varieties. This paper provides scientific data for assessing unintended effects in stacked transgenic plants.