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Dual control of ROS1‐mediated active DNA demethylation by DNA damage‐binding protein 2 (DDB2)
Author(s) -
CórdobaCañero Dolores,
Cognat Valérie,
Ariza Rafael R.,
Roldán Arjona Teresa,
Molinier Jean
Publication year - 2017
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13753
Subject(s) - dna demethylation , dna methylation , ap site , dna repair , dna damage , biology , dna , demethylation , microbiology and biotechnology , chemistry , biochemistry , gene expression , gene
Summary By controlling gene expression, DNA methylation contributes to key regulatory processes during plant development. Genomic methylation patterns are dynamic and must be properly maintained and/or re‐established upon DNA replication and active removal, and therefore require sophisticated control mechanisms. Here we identify direct interplay between the DNA repair factor DNA damage‐binding protein 2 ( DDB 2) and the ROS 1‐mediated active DNA demethylation pathway in Arabidopsis thaliana . We show that DDB 2 forms a complex with ROS 1 and AGO 4 and that they act at the ROS 1 locus to modulate levels of DNA methylation and therefore ROS 1 expression. We found that DDB 2 represses enzymatic activity of ROS 1. DNA demethylation intermediates generated by ROS 1 are processed by the DNA 3′‐phosphatase ZDP and the apurinic/apyrimidinic endonuclease APE 1L, and we also show that DDB 2 interacts with both enzymes and stimulates their activities. Taken together, our results indicate that DDB 2 acts as a critical regulator of ROS 1‐mediated active DNA demethylation.