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MAPK kinase 10.2 promotes disease resistance and drought tolerance by activating different MAPKs in rice
Author(s) -
Ma Haigang,
Chen Jie,
Zhang Zhenzhen,
Ma Ling,
Yang Zeyu,
Zhang Qinglu,
Li Xianghua,
Xiao Jinghua,
Wang Shiping
Publication year - 2017
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13674
Subject(s) - kinase , mapk/erk pathway , resistance (ecology) , plant disease resistance , drought resistance , protein kinase a , microbiology and biotechnology , biology , agronomy , genetics , gene
Summary Mitogen‐activated protein kinase ( MAPK ) cascades, with each cascade consisting of a MAPK kinase kinase ( MAPKKK ), a MAPK kinase ( MAPKK ) and a MAPK , have important roles in different biological processes. However, the signal transduction in rice MAPK cascades remains to be elucidated. We show that the structural non‐canonical MAPKK , MPKK 10.2, enhances rice resistance to Xanthomonas oryzae pv. oryzicola ( Xoc ), which causes bacterial streak disease, and increases rice tolerance to drought stress by phosphorylating and activating two MAPK s, MPK 6 and MPK 3, respectively. MPKK 10.2 ‐overexpressing (oe) plants showed enhanced resistance to both Xoc and drought, whereas MPKK 10.2 ‐ RNA interference ( RNA i) plants had increased sensitivity to both Xoc and drought. MPKK 10.2 physically interacted with MPK 6 and MPK 3, and phosphorylated the two MAPK s in vivo . Transcriptionally modulating MPKK 10.2 influenced MPK 6 phosphorylation during rice‐ Xoc interaction, and MPKK 10.2 ‐oe/ MPK 6 ‐ RNA i double mutants showed increased sensitivity to Xoc . MPKK 10.2 ‐oe/ MPK 3 ‐ RNA i double mutants showed survival rates similar to those of control plants, although the survival rates of MPKK 10.2 transgenic plants changed after drought stress. These results suggest that MPKK 10.2 is a node involved in rice response to biotic and abiotic responses by functioning in the cross‐point of two MAPK cascades leading to Xoc resistance and drought tolerance.