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Regulation of tapetum‐specific A9 promoter by transcription factors At MYB 80, At MYB 1 and At MYB 4 in Arabidopsis thaliana and Nicotiana tabacum
Author(s) -
Verma Neetu,
Burma Pradeep Kumar
Publication year - 2017
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13671
Subject(s) - myb , tapetum , promoter , transcription factor , arabidopsis thaliana , biology , transgene , transcriptional regulation , microbiology and biotechnology , gene , gene expression , genetics , mutant , botany , stamen , pollen , microspore
Summary Tapetum‐specific promoters have been successfully used for developing transgenic‐based pollination control systems. Although several tapetum‐specific promoters have been identified, in‐depth studies on regulation of such promoters are scarce. The present study analyzes the regulation of the A9 promoter, one of the first tapetum‐specific promoter identified in Arabidopsis thaliana . Transcription factors ( TF s) At MYB 80, At MYB 1 (positive regulators) identified by in silico analysis were found to upregulate A9 promoter activity following the over‐expression of the TF s in transient and stable (transgenic) expression assays in both A . thaliana and tobacco. Furthermore, mutations of binding sites of these TF s in the A9 promoter led to loss of its activity. The role of a negative regulator At MYB 4 was also studied by analyzing the activity of A9 promoter following transient expression of RNA i against the TF and by mutating binding sites for At MYB 4 in the A9 promoter. While no changes were observed in case of A . thaliana , the A9 promoter was activated in the roots of transgenic tobacco plants, highlighting the role of these cis‐ elements in keeping the A9 promoter repressed in the roots of tobacco.