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The high‐quality genome of Brassica napus cultivar ‘ ZS 11’ reveals the introgression history in semi‐winter morphotype
Author(s) -
Sun Fengming,
Fan Guangyi,
Hu Qiong,
Zhou Yongming,
Guan Mei,
Tong Chaobo,
Li Jiana,
Du Dezhi,
Qi Cunkou,
Jiang Liangcai,
Liu Weiqing,
Huang Shunmou,
Chen Wenbin,
Yu Jingyin,
Mei Desheng,
Meng Jinling,
Zeng Peng,
Shi Jiaqin,
Liu Kede,
Wang Xi,
Wang Xinfa,
Long Yan,
Liang Xinming,
Hu Zhiyong,
Huang Guodong,
Dong Caihua,
Zhang He,
Li Jun,
Zhang Yaolei,
Li Liangwei,
Shi Chengcheng,
Wang Jiahao,
Lee Simon MingYuen,
Guan Chunyun,
Xu Xun,
Liu Shengyi,
Liu Xin,
Chalhoub Boulos,
Hua Wei,
Wang Hanzhong
Publication year - 2017
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13669
Subject(s) - biology , introgression , vernalization , genome , brassica , cultivar , genetics , genetic diversity , brassica rapa , adaptation (eye) , gene pool , gene , botany , population , demography , neuroscience , sociology
Summary Allotetraploid oilseed rape ( Brassica napus L.) is an agriculturally important crop. Cultivation and breeding of B . napus by humans has resulted in numerous genetically diverse morphotypes with optimized agronomic traits and ecophysiological adaptation. To further understand the genetic basis of diversification and adaptation, we report a draft genome of an Asian semi‐winter oilseed rape cultivar ‘ ZS 11’ and its comprehensive genomic comparison with the genomes of the winter‐type cultivar ‘Darmor‐ bzh ’ as well as two progenitors. The integrated BAC ‐to‐ BAC and whole‐genome shotgun sequencing strategies were effective in the assembly of repetitive regions (especially young long terminal repeats) and resulted in a high‐quality genome assembly of B . napus ‘ ZS 11’. Within a short evolutionary period (~6700 years ago), semi‐winter‐type ‘ ZS 11’ and the winter‐type ‘Darmor‐ bzh ’ maintained highly genomic collinearity. Even so, certain genetic differences were also detected in two morphotypes. Relative to ‘Darmor‐ bzh ’, both two subgenomes of ‘ ZS 11’ are closely related to its progenitors, and the ‘ ZS 11’ genome harbored several specific segmental homoeologous exchanges ( HE s). Furthermore, the semi‐winter‐type ‘ ZS 11’ underwent potential genomic introgressions with B . rapa (A r ). Some of these genetic differences were associated with key agronomic traits. A key gene of A03. FLC 3 regulating vernalization‐responsive flowering time in ‘ ZS 11’ was first experienced HE , and then underwent genomic introgression event with A r , which potentially has led to genetic differences in controlling vernalization in the semi‐winter types. Our observations improved our understanding of the genetic diversity of different B . napus morphotypes and the cultivation history of semi‐winter oilseed rape in Asia.