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Temporal and spatial transcriptomic and micro RNA dynamics of CAM photosynthesis in pineapple
Author(s) -
Wai Ching M.,
VanBuren Robert,
Zhang Jisen,
Huang Lixian,
Miao Wenjing,
Edger Patrick P.,
Yim Won C.,
Priest Henry D.,
Meyers Blake C.,
Mockler Todd,
Smith J. Andrew C.,
Cushman John C.,
Ming Ray
Publication year - 2017
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13630
Subject(s) - crassulacean acid metabolism , transcriptome , biology , diel vertical migration , photosynthesis , ananas , botany , gene , circadian clock , gene expression , genetics , ecology
Summary The altered carbon assimilation pathway of crassulacean acid metabolism ( CAM ) photosynthesis results in an up to 80% higher water‐use efficiency than C 3 photosynthesis in plants making it a potentially useful pathway for engineering crop plants with improved drought tolerance. Here we surveyed detailed temporal (diel time course) and spatial (across a leaf gradient) gene and micro RNA (mi RNA ) expression patterns in the obligate CAM plant pineapple [ Ananas comosus (L.) Merr.]. The high‐resolution transcriptome atlas allowed us to distinguish between CAM ‐related and non‐ CAM gene copies. A differential gene co‐expression network across green and white leaf diel datasets identified genes with circadian oscillation, CAM ‐related functions, and source‐sink relations. Gene co‐expression clusters containing CAM pathway genes are enriched with clock‐associated cis ‐elements, suggesting circadian regulation of CAM . About 20% of pineapple micro RNA s have diel expression patterns, with several that target key CAM ‐related genes. Expression and physiology data provide a model for CAM ‐specific carbohydrate flux and long‐distance hexose transport. Together these resources provide a list of candidate genes for targeted engineering of CAM into C 3 photosynthesis crop species.