Premium
Comparative transcriptome analysis of nodules of two Mesorhizobium –chickpea associations with differential symbiotic efficiency under phosphate deficiency
Author(s) -
Nasr Esfahani Maryam,
Inoue Komaki,
Chu Ha Duc,
Nguyen Kien Huu,
Ha Chien,
Watanabe Yasuko,
Burritt David J.,
HerreraEstrella Luis,
Mochida Keiichi,
Tran LamSon Phan
Publication year - 2017
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13616
Subject(s) - biology , mesorhizobium , transcriptome , legume , symbiosis , metabolomics , nitrogen fixation , root nodule , gene , rhizobia , pi , gene expression , botany , genetics , biochemistry , bioinformatics , bacteria
Summary Phosphate (Pi) deficiency is known to be a major limitation for symbiotic nitrogen fixation ( SNF ), and hence legume crop productivity globally. However, very little information is available on the adaptive mechanisms, particularly in the important legume crop chickpea ( Cicer arietinum L.), which enable nodules to respond to low‐Pi availability. Thus, to elucidate these mechanisms in chickpea nodules at molecular level, we used an RNA sequencing approach to investigate transcriptomes of the nodules in Mesorhizobium mediterraneum SWRI 9–( Mm SWRI 9)–chickpea and M . ciceri CP ‐31–( Mc CP ‐31)–chickpea associations under Pi‐sufficient and Pi‐deficient conditions, of which the Mc CP ‐31–chickpea association has a better SNF capacity than the Mm SWRI 9–chickpea association during Pi starvation. Our investigation revealed that more genes showed altered expression patterns in Mm SWRI 9‐induced nodules than in Mc CP ‐31‐induced nodules (540 vs. 225) under Pi deficiency, suggesting that the Pi‐starvation‐more‐sensitive Mm SWRI 9‐induced nodules required expression change in a larger number of genes to cope with low‐Pi stress than the Pi‐starvation‐less‐sensitive Mc CP ‐31‐induced nodules. The functional classification of differentially expressed genes ( DEG s) was examined to gain an understanding of how chickpea nodules respond to Pi starvation, caused by soil Pi deficiency. As a result, more DEG s involved in nodulation, detoxification, nutrient/ion transport, transcriptional factors, key metabolic pathways, Pi remobilization and signalling were found in Pi‐starved Mm SWRI 9‐induced nodules than in Pi‐starved Mc CP ‐31‐induced nodules. Our findings have enabled the identification of molecular processes that play important roles in the acclimation of nodules to Pi deficiency, ultimately leading to the development of Pi‐efficient chickpea symbiotic associations suitable for Pi‐deficient soils.