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The DNA translocase RAD 5A acts independently of the other main DNA repair pathways, and requires both its ATP ase and RING domain for activity in Arabidopsis thaliana
Author(s) -
Klemm Tobias,
Mannuß Anja,
Kobbe Daniela,
Knoll Alexander,
Trapp Oliver,
Dorn Annika,
Puchta Holger
Publication year - 2017
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13602
Subject(s) - biology , dna repair , arabidopsis , dna damage , microbiology and biotechnology , nucleotide excision repair , mutant , helicase , dna , genetics , gene , rna
Summary Multiple pathways exist to repair DNA damage induced by methylating and crosslinking agents in Arabidopsis thaliana . The SWI 2/ SNF 2 translocase RAD 5A, the functional homolog of budding yeast Rad5 that is required for the error‐free branch of post‐replicative repair, plays a surprisingly prominent role in the repair of both kinds of lesions in Arabidopsis. Here we show that both the ATP ase domain and the ubiquitination function of the RING domain of the Arabidopsis protein are essential for the cellular response to different forms of DNA damage. To define the exact role of RAD 5A within the complex network of DNA repair pathways, we crossed the rad5a mutant line with mutants of different known repair factors of Arabidopsis. We had previously shown that RAD 5A acts independently of two main pathways of replication‐associated DNA repair defined by the helicase RECQ 4A and the endonuclease MUS 81. The enhanced sensitivity of all double mutants tested in this study indicates that the repair of damaged DNA by RAD 5A also occurs independently of nucleotide excision repair (At RAD 1), single‐strand break repair (At PARP 1), as well as microhomology‐mediated double‐strand break repair (At TEB ). Moreover, RAD 5A can partially complement for a deficient At ATM ‐mediated DNA damage response in plants, as the double mutant shows phenotypic growth defects.