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A removable virus vector suitable for plant genome editing
Author(s) -
Chujo Tetsuya,
Yoshikawa Manabu,
Ariga Hirotaka,
Endo Masaki,
Toki Seiichi,
Ishibashi Kazuhiro
Publication year - 2017
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13581
Subject(s) - biology , genome , virus , rna , virology , vector (molecular biology) , plant virus , mutagenesis , expression vector , cucumber mosaic virus , rna virus , genetics , gene , mutation , recombinant dna
Summary Plant genome editing is achieved by the expression of sequence‐specific nucleases ( SSN s). RNA virus vector‐mediated expression of SSN s is a promising approach for transgene integration‐free targeted mutagenesis in plants. However, the removal of virus vectors from infected plants is challenging because no antiviral drugs are available against plant viruses. Here, we developed a removable RNA virus vector that carries the target site of tobacco micro RNA 398 (miR398) whose expression is induced during shoot regeneration. In the inoculated leaves in which expression of miR398 is not induced, insertion of the miR398 target site did not affect the practicability of the virus vector. When shoots were regenerated from the infected leaves, miR398 was expressed and viral RNA was eliminated. The virus vector successfully expressed SSN s in inoculated leaves, from which virus‐free genome‐edited plants were regenerated via tissue culture.