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Global analysis of canola genes targeted by SHORT HYPOCOTYL UNDER BLUE 1 during endosperm and embryo development
Author(s) -
Zhang Huanan,
Cheng Feng,
Xiao Yuguo,
Kang Xiaojun,
Wang Xiaowu,
Kuang Rui,
Ni Min
Publication year - 2017
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13542
Subject(s) - endosperm , biology , canola , gene , embryo , arabidopsis , hypocotyl , arabidopsis thaliana , genetics , botany , microbiology and biotechnology , mutant
Summary Seed development in dicots includes early endosperm proliferation followed by growth of the embryo to replace the endosperm. Endosperm proliferation in dicots not only provides nutrient supplies for subsequent embryo development but also enforces a space limitation, influencing final seed size. Overexpression of Arabidopsis SHORT HYPOCOTYL UNDER BLUE 1::uidA ( SHB 1:uidA ) in canola produces large seeds. We performed global analysis of the canola genes that were expressed and influenced by SHB 1 during early endosperm proliferation at 8 days after pollination ( DAP ) and late embryo development at 13 DAP . Overexpression of SHB 1 altered the expression of 973 genes at 8 DAP and 1035 genes at 13 DAP . We also surveyed the global SHB 1 association sites, and merging of these sites with the RNA sequencing data identified a set of canola genes targeted by SHB 1. The 8‐ DAP list includes positive and negative genes that influence endosperm proliferation and are homologous to Arabidopsis MINI 3 , IKU 2 , SHB 1 , AGL 62 , FIE and AP 2 . We revealed a major role for SHB 1 in canola endosperm development based on the dynamics of SHB 1‐altered gene expression, the magnitude of SHB 1 chromatin immunoprecipitation enrichment and the over‐representation of eight regulatory genes for endosperm development. Our studies focus on an important agronomic trait in a major crop for global agriculture. The datasets on stage‐specific and SHB 1‐induced gene expression and genes targeted by SHB 1 also provide a useful resource in the field of endosperm development and seed size engineering. Our practices in an allotetraploid species will impact similar studies in other crop species.