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Jasmonate inhibits COP 1 activity to suppress hypocotyl elongation and promote cotyledon opening in etiolated Arabidopsis seedlings
Author(s) -
Zheng Yuyu,
Cui Xuefei,
Su Liang,
Fang Shuang,
Chu Jinfang,
Gong Qingqiu,
Yang Jianping,
Zhu Ziqiang
Publication year - 2017
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13539
Subject(s) - hypocotyl , cotyledon , photomorphogenesis , etiolation , arabidopsis , seedling , microbiology and biotechnology , biology , elongation , jasmonate , auxin , phytochrome , botany , mutant , biochemistry , gene , materials science , red light , ultimate tensile strength , metallurgy , enzyme
Summary A germinating seedling undergoes skotomorphogenesis to emerge from the soil and reach for light. During this phase, the cotyledons are closed, and the hypocotyl elongates. Upon exposure to light, the seedling rapidly switches to photomorphogenesis by opening its cotyledons and suppressing hypocotyl elongation. The E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC 1 ( COP 1) is critical for maintaining skotomorphogenesis. Here, we report that jasmonate ( JA ) suppresses hypocotyl elongation and stimulates cotyledon opening in etiolated seedlings, partially phenocopying cop1 mutants in the dark. We also find that JA stabilizes several COP 1‐targeted transcription factors in a COP 1‐dependent manner. RNA ‐seq analysis further defines a JA ‐light co‐modulated and cop1 ‐dependent transcriptome, which is enriched for auxin‐responsive genes and genes participating in cell wall modification. JA suppresses COP 1 activity through at least two distinct mechanisms: decreasing COP 1 protein accumulation in the nucleus; and reducing the physical interaction between COP 1 and its activator, SUPPRESSOR OF PHYTOCHROME A‐105 1 ( SPA 1). Our work reveals that JA suppresses COP 1 activity to stabilize COP 1 targets, thereby inhibiting hypocotyl elongation and stimulating cotyledon unfolding in etiolated Arabidopsis seedlings.