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Eukaryotic translation initiation factor 2B‐beta ( eIF 2B β), a new class of plant virus resistance gene
Author(s) -
Shopan Jannat,
Mou Haipeng,
Zhang Lili,
Zhang Changtong,
Ma Weiwei,
Walsh John A.,
Hu Zhongyuan,
Yang Jinghua,
Zhang Mingfang
Publication year - 2017
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13519
Subject(s) - turnip mosaic virus , biology , potyvirus , eukaryotic translation , genetics , virology , eif4g , eif4e , gene , plant virus , virus , translation (biology) , messenger rna
Summary Recessive resistances to plant viruses in the Potyvirus genus have been found to be based on mutations in the plant eukaryotic translation initiation factors, eIF 4E and eIF 4G or their isoforms. Here we report that natural, monogenic recessive resistance to the Potyvirus Turnip mosaic virus (Tu MV ) has been found in a number of mustard ( Brassica juncea ) accessions. Bulked segregant analysis and sequencing of resistant and susceptible plant lines indicated the resistance is controlled by a single recessive gene, re cessive T u MVr esistance 03 ( retr03 ), an allele of the eukaryotic translation initiation factor 2B‐beta ( eIF 2B β). Silencing of eIF 2B β in a Tu MV ‐susceptible mustard plant line and expression of eIF 2B β from a Tu MV ‐susceptible line in a Tu MV ‐resistant mustard plant line confirmed the new resistance mechanism. A functional copy of a specific allele of eIF 2B β is required for efficient Tu MV infection. eIF 2B β represents a new class of virus resistance gene conferring resistance to any pathogen. eIF 2B acts as a guanine nucleotide exchange factor ( GEF ) for its GTP ‐binding protein partner eIF 2 via interaction with eIF 2· GTP at an early step in translation initiation. Further genotyping indicated that a single non‐synonymous substitution (A120G) in the N‐terminal region of eIF 2B β was responsible for the Tu MV resistance. A reproducible marker has been developed, facilitating marker‐assisted selection for Tu MV resistance in B. juncea . Our findings provide a new target for seeking natural resistance to potyviruses and new opportunities for the control of potyviruses using genome editing techniques targeted on eIF 2B β.