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RAD 54 forms DNA repair foci in response to DNA damage in living plant cells
Author(s) -
Hirakawa Takeshi,
Hasegawa Junko,
White Charles I.,
Matsunaga Sachihiro
Publication year - 2017
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13499
Subject(s) - dna damage , chromatin , dna , dna repair , homologous recombination , microbiology and biotechnology , biology , ataxia telangiectasia , arabidopsis thaliana , chemistry , genetics , gene , mutant
Summary Plants have various defense mechanisms against environmental stresses that induce DNA damage. Genetic and biochemical analyses have revealed the sensing and signaling of DNA damage, but little is known about subnuclear dynamics in response to DNA damage in living plant cells. Here, we observed that the chromatin remodeling factor RAD 54, which is involved in DNA repair via the homologous recombination pathway, formed subnuclear foci (termed RAD 54 foci) in Arabidopsis thaliana after induction of DNA double‐strand breaks. The appearance of RAD 54 foci was dependent on the ATAXIA ‐ TELANGIECTASIA MUTATED – SUPPRESSOR OF GAMMA RESPONSE 1 pathway, and RAD 54 foci were co‐localized with γH2 AX signals. Laser irradiation of a subnuclear area demonstrated that in living cells RAD 54 was specifically accumulated at the damaged site. In addition, the formation of RAD 54 foci showed specificity for cell type and region. We conclude that RAD 54 foci correspond to DNA repair foci in A. thaliana .