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High spatial resolution mass spectrometry imaging reveals the genetically programmed, developmental modification of the distribution of thylakoid membrane lipids among individual cells of maize leaf
Author(s) -
Dueñas Maria Emilia,
Klein Adam T.,
Alexander Liza E.,
YandeauNelson Marna D.,
Nikolau Basil J.,
Lee Young Jin
Publication year - 2017
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13422
Subject(s) - thylakoid , mass spectrometry imaging , mass spectrometry , membrane , biophysics , high resolution , superresolution , chemistry , chloroplast , botany , biology , microbiology and biotechnology , biochemistry , chromatography , remote sensing , gene , geography , computer science , artificial intelligence , image (mathematics)
Summary Metabolism in plants is compartmentalized among different tissues, cells and subcellular organelles. Mass spectrometry imaging ( MSI ) with matrix‐assisted laser desorption ionization ( MALDI ) has recently advanced to allow for the visualization of metabolites at single‐cell resolution. Here we applied 5‐ and 10 μm high spatial resolution MALDI ‐ MSI to the asymmetric Kranz anatomy of Zea mays (maize) leaves to study the differential localization of two major anionic lipids in thylakoid membranes, sulfoquinovosyldiacylglycerols ( SQDG ) and phosphatidylglycerols ( PG ). The quantification and localization of SQDG and PG molecular species, among mesophyll (M) and bundle sheath ( BS ) cells, are compared across the leaf developmental gradient from four maize genotypes (the inbreds B73 and Mo17, and the reciprocal hybrids B73 × Mo17 and Mo17 × B73). SQDG species are uniformly distributed in both photosynthetic cell types, regardless of leaf development or genotype; however, PG shows photosynthetic cell‐specific differential localization depending on the genotype and the fatty acyl chain constituent. Overall, 16:1‐containing PG s primarily contribute to the thylakoid membranes of M cells, whereas BS chloroplasts are mostly composed of 16:0‐containing PG s. Furthermore, PG 32:0 shows genotype‐specific differences in cellular distribution, with preferential localization in BS cells for B73, but more uniform distribution between BS and M cells in Mo17. Maternal inheritance is exhibited within the hybrids, such that the localization of PG 32:0 in B73 × Mo17 is similar to the distribution in the B73 parental inbred, whereas that of Mo17 × B73 resembles the Mo17 parent. This study demonstrates the power of MALDI ‐ MSI to reveal unprecedented insights on metabolic outcomes in multicellular organisms at single‐cell resolution.