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Arabidopsis phosphatidylglycerophosphate phosphatase 1 involved in phosphatidylglycerol biosynthesis and photosynthetic function
Author(s) -
Lin YingChen,
Kobayashi Koichi,
Hung ChunHsien,
Wada Hajime,
Nakamura Yuki
Publication year - 2016
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13311
Subject(s) - biology , chloroplast , arabidopsis , biochemistry , plastid , arabidopsis thaliana , mutant , photosynthesis , phosphatidylglycerol , thylakoid , biosynthesis , microbiology and biotechnology , enzyme , gene , phospholipid , membrane , phosphatidylcholine
Summary Phosphatidylglycerol ( PG ) is an indispensable lipid constituent of photosynthetic membranes, whose function is essential in photosynthetic activity. In higher plants, the biological function of the last step of PG biosynthesis remains elusive because an enzyme catalyzing this reaction step, namely phosphatidylglycerophosphate phosphatase ( PGPP ), has been a missing piece in the entire glycerolipid metabolic map. Here, we report the identification and characterization of At PGPP1 encoding a PGPP in Arabidopsis thaliana . Heterologous expression of At PGPP1 in yeast Δ gep4 complemented growth phenotype and PG ‐producing activity, suggesting that At PGPP1 encodes a functional PGPP . The GUS reporter assay showed that At PGPP1 was preferentially expressed in hypocotyl, vasculatures, trichomes, guard cells, and stigmas. A subcellular localization study with GFP reporter indicated that AtPGPP1 is mainly localized at chloroplasts. A T‐ DNA ‐tagged knockout mutant of At PGPP1 , designated pgpp1‐1 , showed pale green phenotype with reduced PG and chlorophyll contents but no defect in embryo development. In the pgpp1‐1 mutant, ultrastructure of plastids indicated defective development of chloroplasts and measurement of photosynthetic parameters showed impaired photosynthetic activity. These results suggest that AtPGPP1 is a primary plastidic PGPP required for PG biosynthesis and photosynthetic function in Arabidopsis.