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Histone acetyltransferase general control non‐repressed protein 5 ( GCN 5) affects the fatty acid composition of Arabidopsis thaliana seeds by acetylating fatty acid desaturase3 ( FAD 3 )
Author(s) -
Wang Tianya,
Xing Jiewen,
Liu Xinye,
Liu Zhenshan,
Yao Yingyin,
Hu Zhaorong,
Peng Huiru,
Xin Mingming,
Zhou DaoXiu,
Zhang Yirong,
Ni Zhongfu
Publication year - 2016
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13300
Subject(s) - acetylation , arabidopsis , histone , histone acetyltransferase , biochemistry , arabidopsis thaliana , acetyltransferase , mutant , biology , histone acetyltransferases , linoleic acid , fatty acid , chemistry , gene
Summary Seed oils are important natural resources used in the processing and preparation of food. Histone modifications represent key epigenetic mechanisms that regulate gene expression, plant growth and development. However, histone modification events during fatty acid ( FA ) biosynthesis are not well understood. Here, we demonstrate that a mutation of the histone acetyltransferase GCN 5 can decrease the ratio of α‐linolenic acid ( ALA ) to linoleic acid ( LA ) in seed oil. Using RNA ‐Seq and Ch IP assays, we identified FAD 3, LACS 2, LPP 3 and PLAIII β as the targets of GCN 5. Notably, the GCN 5‐dependent H3K9/14 acetylation of FAD 3 determined the expression levels of FAD 3 in Arabidopsi s thaliana seeds, and the ratio of ALA / LA in the gcn5 mutant was rescued to the wild‐type levels through the overexpression of FAD 3 . The results of this study indicated that GCN 5 modulated FA biosynthesis by affecting the acetylation levels of FAD 3 . We provide evidence that histone acetylation is involved in FA biosynthesis in Arabidopsis seeds and might contribute to the optimization of the nutritional structure of edible oils through epigenetic engineering.