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The transcription factors MS 188 and AMS form a complex to activate the expression of CYP 703A2 for sporopollenin biosynthesis in Arabidopsis thaliana
Author(s) -
Xiong ShuangXi,
Lu JieYang,
Lou Yue,
Teng XiaoDong,
Gu JingNan,
Zhang Cheng,
Shi QiangSheng,
Yang ZhongNan,
Zhu Jun
Publication year - 2016
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13284
Subject(s) - tapetum , sporopollenin , microbiology and biotechnology , transcription factor , arabidopsis , biosynthesis , biology , chromatin immunoprecipitation , biochemistry , arabidopsis thaliana , chemistry , mutant , pollen , gene expression , botany , enzyme , gene , stamen , microspore , promoter
Summary The sexine layer of pollen grain is mainly composed of sporopollenins. The sporophytic secretory tapetum is required for the biosynthesis of sporopollenin. Although several enzymes involved in sporopollenin biosynthesis have been reported, the regulatory mechanism of these enzymes in tapetal layer remains elusive. ABORTED MICROSPORES ( AMS ) and MALE STERILE 188 / MYB 103 / MYB 80 ( MS 188 / MYB 103 / MYB 80 ) are two tapetal cell‐specific transcription factors required for pollen wall formation. AMS functions upstream of MS 188 . Here we report that AMS and MS 188 target the CYP 703A2 gene, which is involved in sporopollenin biosynthesis. We found that AMS and MS 188 were localized in tapetum while CYP 703A2 was localized in both tapetum and locule. Chromatin immunoprecipitation (Ch IP ) showed that MS 188 directly bound to the promoter of CYP 703A2 and luciferase‐inducible assay showed that MS 188 activated the expression of CYP 703A2 . Yeast two‐hybrid and electrophoretic mobility shift assays ( EMSA s) further demonstrated that MS 188 complexed with AMS . The expression of CYP 703A2 could be partially restored by the elevated levels of MS 188 in the ams mutant. Therefore, our data reveal that MS 188 coordinates with AMS to activate CYP 703A2 in sporopollenin biosynthesis of plant tapetum.