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At RAD 5A is a DNA translocase harboring a HIRAN domain which confers binding to branched DNA structures and is required for DNA repair in vivo
Author(s) -
Kobbe Daniela,
Kahles Andy,
Walter Maria,
Klemm Tobias,
Mannuss Anja,
Knoll Alexander,
Focke Manfred,
Puchta Holger
Publication year - 2016
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13283
Subject(s) - biology , translocase , dna replication , replication protein a , dna repair , arabidopsis , dna , genetics , microbiology and biotechnology , hmg box , dna binding protein , biochemistry , gene , mutant , transcription factor , chromosomal translocation
Summary DNA lesions such as crosslinks represent obstacles for the replication machinery. Nonetheless, replication can proceed via the DNA damage tolerance pathway also known as postreplicative repair pathway. SNF 2 ATP ase Rad5 homologs, such as RAD 5A of the model plant Arabidopsis thaliana , are important for the error‐free mode of this pathway. We able to demonstrate before, that RAD 5A is a key factor in the repair of DNA crosslinks in Arabidopsis. Here, we show by in vitro analysis that At RAD 5A protein is a DNA translocase able to catalyse fork regression. Interestingly, replication forks with a gap in the leading strand are processed best, in line with its suggested function. Furthermore At RAD 5A catalyses branch migration of a Holliday junction and is furthermore not impaired by the DNA binding of a model protein, which is indicative of its ability to displace other proteins. Rad5 homologs possess HIRAN (Hip116, Rad5; N‐terminal) domains. By biochemical analysis we were able to demonstrate that the HIRAN domain variant from Arabidopsis RAD 5A mediates structure selective DNA binding without the necessity for a free 3′ OH group as has been shown to be required for binding of HIRAN domains in a mammalian RAD 5 homolog. The biological importance of the HIRAN domain in At RAD 5A is demonstrated by our result that it is required for its function in DNA crosslink repair in vivo .