Premium
The importance of SERINE DECARBOXYLASE 1 ( SDC 1) and ethanolamine biosynthesis during embryogenesis of Arabidopsis thaliana
Author(s) -
Yunus Ian Sofian,
Liu Yuchi,
Nakamura Yuki
Publication year - 2016
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13278
Subject(s) - arabidopsis , ethanolamine , serine , biosynthesis , biochemistry , arabidopsis thaliana , biology , phosphatidylethanolamine , silique , zebrafish , microbiology and biotechnology , phosphatidylcholine , mutant , enzyme , phospholipid , gene , membrane
Summary In plants, ethanolamine is considered a precursor for the synthesis of choline, which is an essential dietary nutrient for animals. An enzyme serine decarboxylase ( SDC ) has been identified and characterized in Arabidopsis, which directly converts serine to ethanolamine, a precursor to phosphorylethanolamine and its subsequent metabolites in plants. However, the importance of SDC and ethanolamine production in plant growth and development remains unclear. Here, we show that SDC is required for ethanolamine biosynthesis in vivo and essential in plant embryogenesis in Arabidopsis. The knockout of SDC 1 caused an embryonic lethal defect due to the developmental arrest of the embryos at the heart stage. During embryo development, the expression was observed at the later stages, at which developmental defect occurred in the knockout mutant. Overexpression of SDC1 in planta increased levels of ethanolamine, phosphatidylethanolamine, and phosphatidylcholine both in leaves and siliques. These results suggest that SDC 1 plays an essential role in ethanolamine biosynthesis during the embryogenesis in Arabidopsis.