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Cell cycle‐regulated PLEIADE /At MAP 65‐3 links membrane and microtubule dynamics during plant cytokinesis
Author(s) -
Steiner Alexander,
Rybak Katarzyna,
Altmann Melina,
McFarlane Heather E.,
Klaeger Susan,
Nguyen Ngoc,
Facher Eva,
Ivakov Alexander,
Wanner Gerhard,
Kuster Bernhard,
Persson Staffan,
Braun Pascal,
Hauser MarieTheres,
Assaad Farhah F.
Publication year - 2016
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13275
Subject(s) - cytokinesis , phragmoplast , biology , microbiology and biotechnology , cell plate , microtubule , septin , cell division , cell cycle , microtubule organizing center , kinesin , cell , genetics , centrosome
Summary Cytokinesis, the partitioning of the cytoplasm following nuclear division, requires extensive coordination between cell cycle cues, membrane trafficking and microtubule dynamics. Plant cytokinesis occurs within a transient membrane compartment known as the cell plate, to which vesicles are delivered by a plant‐specific microtubule array, the phragmoplast. While membrane proteins required for cytokinesis are known, how these are coordinated with microtubule dynamics and regulated by cell cycle cues remains unclear. Here, we document physical and genetic interactions between Transport Protein Particle II ( TRAPPII ) tethering factors and microtubule‐associated proteins of the PLEIADE /At MAP 65 family. These interactions do not specifically affect the recruitment of either TRAPPII or MAP 65 proteins to the cell plate or midzone. Rather, and based on single versus double mutant phenotypes, it appears that they are required to coordinate cytokinesis with the nuclear division cycle. As MAP 65 family members are known to be targets of cell cycle‐regulated kinases, our results provide a conceptual framework for how membrane and microtubule dynamics may be coordinated with each other and with the nuclear cycle during plant cytokinesis.