Requirement for flap endonuclease 1 (  FEN 1 ) to maintain genomic stability and transcriptional gene silencing in Arabidopsis | Zendy

Zhang Jixiang | Zendy; Xie Shaojun | Zendy; Zhu JianKang | Zendy; Gong Zhizhong | Zendy

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Requirement for flap endonuclease 1 ( FEN 1 ) to maintain genomic stability and transcriptional gene silencing in Arabidopsis

Author(s) -

Zhang Jixiang,

Xie Shaojun,

Zhu JianKang,

Gong Zhizhong

Publication year - 2016

Publication title -

the plant journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.058

H-Index - 269

eISSN - 1365-313X

pISSN - 0960-7412

DOI - 10.1111/tpj.13224

Subject(s) - arabidopsis , biology , okazaki fragments , gene , gene silencing , genetics , mutant , genome instability , chromatin , chromatin immunoprecipitation , endonuclease , microbiology and biotechnology , gene expression , promoter , dna , dna repair , dna damage , eukaryotic dna replication

Summary As a central component in the maturation of Okazaki fragments, flap endonuclease 1 ( FEN 1) removes the 5′‐flap and maintains genomic stability. Here, FEN 1 was cloned as a suppressor of transcriptional gene silencing ( TGS ) from a forward genetic screen. FEN 1 is abundant in the root and shoot apical meristems and FEN 1‐ GFP shows a nucleolus‐localized signal in tobacco cells. The Arabidopsis fen1‐1 mutant is hypersensitive to methyl methanesulfonate and shows reduced telomere length. Interestingly, genome‐wide chromatin immunoprecipitation and RNA sequencing results demonstrate that FEN 1 mutation leads to a decrease in the level of H3K27me3 and an increase in the expression of a subset of genes marked with H3K27me3. Overall, these results uncover a role for FEN 1 in mediating TGS as well as maintaining genome stability in Arabidopsis.

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