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Requirement for flap endonuclease 1 ( FEN 1 ) to maintain genomic stability and transcriptional gene silencing in Arabidopsis
Author(s) -
Zhang Jixiang,
Xie Shaojun,
Zhu JianKang,
Gong Zhizhong
Publication year - 2016
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13224
Subject(s) - arabidopsis , biology , okazaki fragments , gene , gene silencing , genetics , mutant , genome instability , chromatin , chromatin immunoprecipitation , endonuclease , microbiology and biotechnology , gene expression , promoter , dna , dna repair , dna damage , eukaryotic dna replication
Summary As a central component in the maturation of Okazaki fragments, flap endonuclease 1 ( FEN 1) removes the 5′‐flap and maintains genomic stability. Here, FEN 1 was cloned as a suppressor of transcriptional gene silencing ( TGS ) from a forward genetic screen. FEN 1 is abundant in the root and shoot apical meristems and FEN 1‐ GFP shows a nucleolus‐localized signal in tobacco cells. The Arabidopsis fen1‐1 mutant is hypersensitive to methyl methanesulfonate and shows reduced telomere length. Interestingly, genome‐wide chromatin immunoprecipitation and RNA sequencing results demonstrate that FEN 1 mutation leads to a decrease in the level of H3K27me3 and an increase in the expression of a subset of genes marked with H3K27me3. Overall, these results uncover a role for FEN 1 in mediating TGS as well as maintaining genome stability in Arabidopsis.