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Root‐specific expression of opine genes and opine accumulation in some cultivars of the naturally occurring genetically modified organism Nicotiana tabacum
Author(s) -
Chen Ke,
Borne François Dorlhac,
Julio Emilie,
Obszynski Julie,
Pale Patrick,
Otten Léon
Publication year - 2016
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13196
Subject(s) - nicotiana tabacum , biology , agrobacterium , gene , nicotiana benthamiana , genetics , nicotiana , gene expression , transformation (genetics) , botany , solanaceae
Summary Previous studies have shown that Nicotiana tabacum contains three Agrobacterium ‐derived T‐ DNA sequences inherited from its paternal ancestor Nicotiana tomentosiformis . Among these, the TB locus carries an intact mannopine synthase 2′ gene ( TB ‐ mas2 ′). This gene is similar to the Agrobacterium rhizogenes A4‐ mas2 ′ gene that encodes the synthesis of the Amadori compound deoxyfructosyl‐glutamine ( DFG or santhopine). In this study we show that TB ‐ mas2 ′ is expressed at very low levels in N. tomentosiformis and in most N. tabacum cultivars; however, some cultivars show high TB ‐ mas2 ′ expression levels. The TB ‐ mas2 ′ promoter sequences of low‐ and high‐expressing cultivars are identical. The low/high level of expression segregates as a single Mendelian factor in a cross between a low‐ and a high‐expression cultivar. pTB ‐mas2 ′ ‐ GUS and pA 4‐mas2 ′ ‐ GUS reporter genes were stably introduced in N. benthamiana . Both were mainly expressed in the root expansion zone and leaf vasculature. Roots of tobacco cultivars with high TB ‐ mas2 ′ expression contain detectable levels of DFG .