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Solanum pennellii backcross inbred lines ( BIL s) link small genomic bins with tomato traits
Author(s) -
Ofner Itai,
Lashbrooke Justin,
Pleban Tzili,
Aharoni Asaph,
Zamir Dani
Publication year - 2016
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13194
Subject(s) - introgression , backcrossing , biology , inbred strain , solanum , quantitative trait locus , population , genetics , botany , gene , demography , sociology
Summary We present a resource for fine mapping of traits derived from the wild tomato species Solanum pennellii ( LA 0716). The population of backcross inbred lines ( BIL s) is composed of 446 lines derived after a few generations of backcrosses of the wild species with cultivated tomato (cultivar M82; LA 3475), followed by more than seven generations of self‐pollination. The BIL s were genotyped using the 10K SOL ‐ CAP single nucleotide polymorphism ( SNP ) ‐Chip, and 3700 polymorphic markers were used to map recombination break points relative to the physical map of Solanum lycopersicum . The BIL s carry, on average, 2.7 introgressions per line, with a mean introgression length of 11.7 Mbp. Whereas the classic 76 introgression lines ( IL s) partitioned the genome into 106 mapping bins, the BIL s generated 633 bins, thereby enhancing the mapping resolution of traits derived from the wild species. We demonstrate the power of the BIL s for rapid fine mapping of simple and complex traits derived from the wild tomato species.

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