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Non‐specific phospholipase C1 affects silicon distribution and mechanical strength in stem nodes of rice
Author(s) -
Cao Huasheng,
Zhuo Lin,
Su Yuan,
Sun Linxiao,
Wang Xuemin
Publication year - 2016
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13165
Subject(s) - phosphatidic acid , vascular bundle , hemicellulose , chemistry , oryza sativa , microbiology and biotechnology , silicon , biochemistry , botany , biophysics , biology , cellulose , membrane , gene , phospholipid , organic chemistry
Summary Silicon, the second abundant element in the crust, is beneficial for plant growth, mechanical strength, and stress responses. Here we show that manipulation of the non‐specific phospholipase C1, NPC 1, alters silicon content in nodes and husks of rice ( Oryza sativa ). Silicon content in NPC 1 ‐overexpressing ( OE ) plants was decreased in nodes but increased in husks compared to wild‐type, whereas RNA i suppression of NPC 1 resulted in the opposite changes to those of NPC 1‐ OE plants. NPC 1 from rice hydrolyzed phospholipids and galactolipids to generate diacylglycerol that can be phosphorylated to phosphatidic acid. Phosphatidic acid interacts with Lsi6, a silicon transporter that is expressed at the highest level in nodes. In addition, the node cells of NPC 1 ‐ OE plants have lower contents of cellulose and hemicellulose, and thinner sclerenchyma and vascular bundle fibre cells than wild‐type plants; whereas NPC 1‐ RNA i plants displayed the opposite changes. These data indicate that NPC 1 modulates silicon distribution and secondary cell wall deposition in nodes and grains, affecting mechanical strength and seed shattering.