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A versatile Multisite Gateway‐compatible promoter and transgenic line collection for cell type‐specific functional genomics in Arabidopsis
Author(s) -
MarquèsBueno Maria Mar,
Morao Ana K.,
Cayrel Anne,
Platre Matthieu P.,
Barberon Marie,
Caillieux Erwann,
Colot Vincent,
Jaillais Yvon,
Roudier François,
Vert Grégory
Publication year - 2016
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13099
Subject(s) - arabidopsis , biology , multicellular organism , cell type , transgene , functional genomics , computational biology , microbiology and biotechnology , gene expression profiling , promoter , gene knockdown , gene expression , gene , genetics , cell , genomics , mutant , genome
Summary Multicellular organisms are composed of many cell types that acquire their specific fate through a precisely controlled pattern of gene expression in time and space dictated in part by cell type‐specific promoter activity. Understanding the contribution of highly specialized cell types in the development of a whole organism requires the ability to isolate or analyze different cell types separately. We have characterized and validated a large collection of root cell type‐specific promoters and have generated cell type‐specific marker lines. These benchmarked promoters can be readily used to evaluate cell type‐specific complementation of mutant phenotypes, or to knockdown gene expression using targeted expression of artificial mi RNA . We also generated vectors and characterized transgenic lines for cell type‐specific induction of gene expression and cell type‐specific isolation of nuclei for RNA and chromatin profiling. Vectors and seeds from transgenic Arabidopsis plants will be freely available, and will promote rapid progress in cell type‐specific functional genomics. We demonstrate the power of this promoter set for analysis of complex biological processes by investigating the contribution of root cell types in the IRT 1‐dependent root iron uptake. Our findings revealed the complex spatial expression pattern of IRT 1 in both root epidermis and phloem companion cells and the requirement for IRT 1 to be expressed in both cell types for proper iron homeostasis.