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MYBD employed by HY 5 increases anthocyanin accumulation via repression of MYBL 2 in Arabidopsis
Author(s) -
Nguyen Nguyen Hoai,
Jeong Chan Young,
Kang GeunHo,
Yoo SangDong,
Hong SukWhan,
Lee Hojoung
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13077
Subject(s) - photomorphogenesis , psychological repression , transcription factor , arabidopsis , microbiology and biotechnology , repressor , biology , myb , phytochrome , hypocotyl , mutant , genetics , botany , gene expression , gene , red light
Summary Photomorphogenesis is an essential program in plant development. This process is effected by the balanced cooperation of many factors under light and dark conditions. In a previous study, we showed that MYB hypocotyl elongation‐related (MYBH) is involved in cell elongation. To expand our understanding of MYBH function, we performed a yeast two‐hybrid assay and identified an MYB‐like Domain transcription factor ( MYBD ). In this study, we investigated the function of MYBD , which is an MYBH homolog involved in anthocyanin accumulation. MYBD expression increased in response to light or cytokinin, and MYBD enhanced anthocyanin biosynthesis via repression of MYBL 2 , which encodes a transcription factor that has a negative effect on this process. In addition, MYBD binding in vivo to the MYBL 2 promoter and the lower level of histone H3K9 acetylation at the upstream region of MYBL 2 in MYBD over‐expressing plants in comparison with wild‐type plants imply that MYBD represses MYBL 2 expression via an epigenetic mechanism. HY 5 directly binds to the MYBD promoter, which indicates that MYBD acts on HY 5‐downstream in light‐ or cytokinin‐triggered signaling pathways, leading to anthocyanin accumulation. Our results suggest that, although MYBD and MYBH are homologs, they act in opposite ways during plant photomorphogenesis, and these functions should be examined in further studies.