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The RNA helicase, eIF 4A‐1, is required for ovule development and cell size homeostasis in Arabidopsis
Author(s) -
Bush Maxwell S.,
Crowe Natalie,
Zheng Tao,
Doonan John H.
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13062
Subject(s) - biology , microbiology and biotechnology , cell cycle , arabidopsis , rna helicase a , meristem , ovule , mutant , mitosis , cell division , eif4a1 , cell growth , initiation factor , helicase , cell , rna , translation (biology) , messenger rna , genetics , gene , embryo
Summary eIF 4A is a highly conserved RNA ‐stimulated ATP ase and helicase involved in the initiation of mRNA translation. The Arabidopsis genome encodes two isoforms, one of which ( eIF 4A‐1) is required for the coordination between cell cycle progression and cell size. A T‐ DNA mutant eif4a1 line, with reduced eIF 4A protein levels, displays slow growth, reduced lateral root formation, delayed flowering and abnormal ovule development. Loss of eIF 4A‐1 reduces the proportion of mitotic cells in the root meristem and perturbs the relationship between cell size and cell cycle progression. Several cell cycle reporter proteins, particularly those expressed at G2/M, have reduced expression in eif4a1 mutant meristems. Single eif4a1 mutants are semisterile and show aberrant ovule growth, whereas double eif4a1 eif4a2 homozygous mutants could not be recovered, indicating that eIF 4A function is essential for plant growth and development.