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Genome‐wide analysis on Chlamydomonas reinhardtii reveals the impact of hydrogen peroxide on protein stress responses and overlap with other stress transcriptomes
Author(s) -
Blaby Ian K.,
BlabyHaas Crysten E.,
PérezPérez María Esther,
Schmollinger Stefan,
FitzGibbon Sorel,
Lemaire Stéphane D.,
Merchant Sabeeha S.
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13053
Subject(s) - chlamydomonas reinhardtii , transcriptome , biology , chloroplast , photosynthesis , reactive oxygen species , gene , chlamydomonas , context (archaeology) , microbiology and biotechnology , gene expression , biochemistry , genetics , paleontology , mutant
Summary Reactive oxygen species ( ROS ) are produced by and have the potential to be damaging to all aerobic organisms. In photosynthetic organisms, they are an unavoidable byproduct of electron transfer in both the chloroplast and mitochondrion. Here, we employ the reference unicellular green alga Chlamydomonas reinhardtii to identify the effect of H 2 O 2 on gene expression by monitoring the changes in the transcriptome in a time‐course experiment. Comparison of transcriptomes from cells sampled immediately prior to the addition of H 2 O 2 and 0.5 and 1 h subsequently revealed 1278 differentially abundant transcripts. Of those transcripts that increase in abundance, many encode proteins involved in ROS detoxification, protein degradation and stress responses, whereas among those that decrease are transcripts encoding proteins involved in photosynthesis and central carbon metabolism. In addition to these transcriptomic adjustments, we observe that addition of H 2 O 2 is followed by an accumulation and oxidation of the total intracellular glutathione pool, and a decrease in photosynthetic O 2 output. Additionally, we analyze our transcriptomes in the context of changes in transcript abundance in response to singlet O 2 ( O 2 * ), and relate our H 2 O 2 ‐induced transcripts to a diurnal transcriptome, where we demonstrate enrichments of H 2 O 2 ‐induced transcripts early in the light phase, late in the light phase and 2 h prior to light. On this basis several genes that are highlighted in this work may be involved in previously undiscovered stress remediation pathways or acclimation responses.