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SCARECROW ‐ LIKE 23 and SCARECROW jointly specify endodermal cell fate but distinctly control SHORT ‐ ROOT movement
Author(s) -
Long Yuchen,
Goedhart Joachim,
Schneijderberg Martinus,
Terpstra Inez,
Shimotohno Akie,
Bouchet Benjamin P.,
Akhmanova Anna,
Gadella Theodorus W. J.,
Heidstra Renze,
Scheres Ben,
Blilou Ikram
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13038
Subject(s) - stele , meristem , cell fate determination , endodermis , transcription factor , microbiology and biotechnology , arabidopsis , biology , cell , botany , genetics , mutant , gene
Summary Intercellular signaling through trafficking of regulatory proteins is a widespread phenomenon in plants and can deliver positional information for the determination of cell fate. In the Arabidopsis root meristem, the cell fate determinant SHORT ‐ ROOT ( SHR ), a GRAS domain transcription factor, acts as a signaling molecule from the stele to the adjacent layer to specify endodermal cell fate. Upon exiting the stele, SHR activates another GRAS domain transcription factor, SCARCROW ( SCR ), which, together with several BIRD / INDETERMINATE DOMAIN proteins, restricts movement of SHR to define a single cell layer of endodermis. Here we report that endodermal cell fate also requires the joint activity of both SCR and its closest homologue SCARECROW ‐ LIKE 23 ( SCL 23). We show that SCL 23 protein moves with zonation‐dependent directionality. Within the meristem, SCL 23 exhibits short‐ranged movement from ground tissue to vasculature. Away from the meristem, SCL 23 displays long‐range rootward movement into meristematic vasculature and a bidirectional radial spread, respectively. As a known target of SHR and SCR , SCL 23 also interacts with SCR and SHR and can restrict intercellular outspread of SHR without relying on nuclear retention as SCR does. Collectively, our data show that SCL 23 is a mobile protein that controls movement of SHR and acts redundantly with SCR to specify endodermal fate in the root meristem.