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The wheat transcription factor Ta GAM yb recruits histone acetyltransferase and activates the expression of a high‐molecular‐weight glutenin subunit gene
Author(s) -
Guo Weiwei,
Yang Hua,
Liu Yongqiang,
Gao Yujiao,
Ni Zhongfu,
Peng Huiru,
Xin Mingming,
Hu Zhaorong,
Sun Qixin,
Yao Yingyin
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.13003
Subject(s) - glutenin , histone acetyltransferase , biology , promoter , chromatin immunoprecipitation , endosperm , arabidopsis , histone , histone h4 , gene , microbiology and biotechnology , protein subunit , gene expression , genetics , mutant
Summary Glutenin proteins in wheat ( Triticum aestivum L.) flour confer unique viscoelastic properties to dough products and, therefore, the concentration and composition of the glutenin proteins determine its end‐use value. However, the mechanisms governing the glutenin gene expression remain elusive. In this study, we report that wheat Ta GAM yb activates the high‐molecular‐weight glutenin subunit genes ( Ta GLU ) through recruiting the histone acetyltransferase GCN 5. By sequencing the promoters of Ta GLU ‐1 genes from 40 modern wheat cultivars, we identified eight types of Ta GAM yb binding motifs and verified these by electrophoretic mobility shift assays. The number of Ta GAM yb binding motifs in Ta GLU ‐1 genes is correlated with the abundance of glutenin in different cultivars. Chromatin immunoprecipitation plus polymerase chain reaction (Ch IP ‐ PCR ) analysis reveals that Ta GCN 5 directly targets the promoters of Ta GLU ‐1 genes in wheat endosperm. We find that Ta GAM yb physically interacts with the wheat histone acetyltransferase Ta GCN 5 and also interacts with Arabidopsis thaliana At GCN 5. Ta GAM yb ectopically expressed in Arabidopsis binds to the Ta GLU ‐1Dy promoter on a Ta GLU ‐1Dy transgene and activates its expression. At GCN 5 also targets the Ta GLU ‐1Dy transgene and is involved in the establishment of acetylation at H3K9 and H3K14. These results demonstrate that Ta GAM yb plays a dual role in activating expression of glutenin gene by directly binding to the Ta GLU promoter and by recruiting GCN 5 to modulate histone acetylation during wheat endosperm development.