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E3 ubiquitin ligase gene CMPG 1–V from Haynaldia villosa L. contributes to powdery mildew resistance in common wheat ( Triticum aestivum L.)
Author(s) -
Zhu Yanfei,
Li Yingbo,
Fei Fei,
Wang Zongkuan,
Wang Wei,
Cao Aizhong,
Liu Yuan,
Han Shuang,
Xing Liping,
Wang Haiyan,
Chen Wei,
Tang Sanyuan,
Huang Xiahe,
Shen Qianhua,
Xie Qi,
Wang Xiue
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12966
Subject(s) - powdery mildew , blumeria graminis , biology , common wheat , ubiquitin ligase , genetics , plant disease resistance , botany , microbiology and biotechnology , gene , ubiquitin , chromosome
Summary Powdery mildew is one of the most devastating wheat fungal diseases. A diploid wheat relative, Haynaldia villosa L., is highly resistant to powdery mildew, and its genetic resource of resistances, such as the Pm21 locus, is now widely used in wheat breeding. Here we report the cloning of a resistance gene from H. villosa , designated CMPG 1–V , that encodes a U–box E3 ubiquitin ligase. Expression of the CMPG 1–V gene was induced in the leaf and stem of H. villosa upon inoculation with Blumeria graminis f. sp. tritici ( Bgt ) fungus, and the presence of Pm21 is essential for its rapid induction of expression. CMPG 1–V has conserved key residues for E3 ligase, and possesses E3 ligase activity in vitro and in vivo . CMPG 1–V is localized in the nucleus, endoplasmic reticulum, plasma membrane and partially in trans ‐Golgi network/early endosome vesicles. Transgenic wheat over‐expressing CMPG 1–V showed improved broad‐spectrum powdery mildew resistance at seedling and adult stages, associated with an increase in expression of salicylic acid‐responsive genes, H 2 O 2 accumulation, and cell‐wall protein cross‐linking at the Bgt infection sites, and the expression of CMPG 1–V in H. villosa was increased when treated with salicylic acid, abscisic acid and H 2 O 2 . These results indicate the involvement of E3 ligase in defense responses to Bgt fungus in wheat, particularly in broad‐spectrum disease resistance, and suggest association of reactive oxidative species and the phytohormone pathway with CMPG 1 – V ‐mediated powdery mildew resistance.