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The Arabidopsis TAC Position Viewer: a high‐resolution map of transformation‐competent artificial chromosome ( TAC ) clones aligned with the Arabidopsis thaliana Columbia‐0 genome
Author(s) -
Hirose Yoshitsugu,
Suda Kunihiro,
Liu YaoGuang,
Sato Shusei,
Nakamura Yukino,
Yokoyama Koji,
Yamamoto Naoki,
Hanano Shigeru,
Takita Eiji,
Sakurai Nozomu,
Suzuki Hideyuki,
Nakamura Yasukazu,
Kaneko Takakazu,
Yano Kentaro,
Tabata Satoshi,
Shibata Daisuke
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12949
Subject(s) - arabidopsis thaliana , arabidopsis , transformation (genetics) , genome , position (finance) , chromosome , biology , genetics , gene , mutant , business , finance
Summary We present a high‐resolution map of genomic transformation‐competent artificial chromosome ( TAC ) clones extending over all Arabidopsis thaliana (Arabidopsis) chromosomes. The Arabidopsis genomic TAC clones have been valuable genetic tools. Previously, we constructed an Arabidopsis genomic TAC library consisting of more than 10 000 TAC clones harboring large genomic DNA fragments extending over the whole Arabidopsis genome. Here, we determined 13 577 end sequences from 6987 Arabidopsis TAC clones and mapped 5937 TAC clones to precise locations, covering approximately 90% of the Arabidopsis chromosomes. We present the large‐scale data set of TAC clones with high‐resolution mapping information as a Java application tool, the Arabidopsis TAC Position Viewer, which provides ready‐to‐go transformable genomic DNA clones corresponding to certain loci on Arabidopsis chromosomes. The TAC clone resources will accelerate genomic DNA cloning, positional walking, complementation of mutants and DNA transformation for heterologous gene expression.