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Increased glutathione contributes to stress tolerance and global translational changes in Arabidopsis
Author(s) -
Cheng MeiChun,
Ko Ko,
Chang WanLing,
Kuo WenChieh,
Chen GuanHong,
Lin TsanPiao
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12940
Subject(s) - glutathione , arabidopsis , jasmonic acid , reactive oxygen species , abiotic stress , abscisic acid , transcriptome , microbiology and biotechnology , auxin , biochemistry , biology , translation (biology) , chemistry , gene expression , gene , messenger rna , enzyme , mutant
Summary Although glutathione is well known for its reactive oxygen species ( ROS ) scavenging function and plays a protective role in biotic stress, its regulatory function in abiotic stress still remains to be elucidated. Our previous study showed that exogenously applied reduced glutathione ( GSH ) could improve abiotic stress tolerance in Arabidopsis. Here, we report that endogenously increased GSH also conferred tolerance to drought and salt stress in Arabidopsis. Moreover, both exogenous and endogenous GSH delayed senescence and flowering time. Polysomal profiling results showed that global translation was enhanced after GSH treatment and by the induced increase of GSH level by salt stress. By performing transcriptomic analyses of steady‐state and polysome‐bound mRNA s in GSH ‐treated plants, we reveal that GSH has a substantial impact on translation. Translational changes induced by GSH treatment target numerous hormones and stress signaling molecules, which might contribute to the enhanced stress tolerance in GSH ‐treated plants. Our translatome analysis also revealed that abscisic acid ( ABA ), auxin and jasmonic acid ( JA ) biosynthesis, as well as signaling genes, were activated during GSH treatment, which has not been reported in previously published transcriptomic data. Together, our data suggest that the increased glutathione level results in stress tolerance and global translational changes.