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The auxin transporter, Os AUX 1, is involved in primary root and root hair elongation and in Cd stress responses in rice ( Oryza sativa L.)
Author(s) -
Yu ChenLiang,
Sun ChenDong,
Shen Chenjia,
Wang Suikang,
Liu Fang,
Liu Yan,
Chen YunLong,
Li Chuanyou,
Qian Qian,
Aryal Bibek,
Geisler Markus,
Jiang De An,
Qi YanHua
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12929
Subject(s) - auxin , root hair , mutant , elongation , wild type , microbiology and biotechnology , oryza sativa , lateral root , reactive oxygen species , biology , chemistry , biochemistry , arabidopsis , gene , materials science , ultimate tensile strength , metallurgy
Summary Auxin and cadmium (Cd) stress play critical roles during root development. There are only a few reports on the mechanisms by which Cd stress influences auxin homeostasis and affects primary root ( PR ) and lateral root ( LR ) development, and almost nothing is known about how auxin and Cd interfere with root hair ( RH ) development. Here, we characterize rice osaux1 mutants that have a longer PR and shorter RH s in hydroponic culture, and that are more sensitive to Cd stress compared to wild‐type (Dongjin). Os AUX 1 expression in root hair cells is different from that of its paralogous gene, At AUX 1 , which is expressed in non‐hair cells. However, Os AUX 1, like At AUX 1, localizes at the plasma membrane and appears to function as an auxin tranporter. Decreased auxin distribution and contents in the osaux1 mutant result in reduction of OsCy CB 1;1 expression and shortened PR s, LR s and RH s under Cd stress, but may be rescued by treatment with the membrane‐permeable auxin 1‐naphthalene acetic acid. Treatment with the auxin transport inhibitors 1‐naphthoxyacetic acid and N ‐1‐naphthylphthalamic acid increased the Cd sensitivity of WT rice. Cd contents in the osaux1 mutant were not altered, but reactive oxygen species‐mediated damage was enhanced, further increasing the sensitivity of the osaux1 mutant to Cd stress. Taken together, our results indicate that Os AUX 1 plays an important role in root development and in responses to Cd stress.