Premium
Regulation of flowering time by the histone deacetylase HDA 5 in A rabidopsis
Author(s) -
Luo Ming,
Tai Ready,
Yu ChunWei,
Yang Songguang,
Chen ChiaYang,
Lin WenDar,
Schmidt Wolfgang,
Wu Keqiang
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12868
Subject(s) - chromatin immunoprecipitation , histone deacetylase , acetylation , chromatin , mutant , biology , histone , hdac11 , histone methyltransferase , histone deacetylase 2 , microbiology and biotechnology , histone h2a , histone h3 , histone deacetylase 5 , complementation , repressor , gene expression , gene , genetics , promoter
Summary The acetylation level of histones on lysine residues regulated by histone acetyltransferases and histone deacetylases plays an important but under‐studied role in the control of gene expression in plants. With the aim of characterizing the Arabidopsis RPD 3/ HDA 1 family histone deacetylase HDA 5, we present evidence showing that HDA 5 displays deacetylase activity. Mutants defective in the expression of HDA 5 displayed a late‐flowering phenotype. Expression of the flowering repressor genes FLC and MAF 1 was up‐regulated in hda5 mutants. Furthermore, the gene activation markers, histone H3 acetylation and H3K4 trimethylation on FLC and MAF 1 chromatin were increased in hda5 ‐ 1 mutants. Chromatin immunoprecipitation analysis showed that HDA 5 binds to the chromatin of FLC and MAF 1 . Bimolecular fluorescence complementation assays and co‐immunoprecipitation assays showed that HDA 5 interacts with FVE , FLD and HDA 6, indicating that these proteins are present in a protein complex involved in the regulation of flowering time. Comparing gene expression profiles of hda5 and hda6 mutants by RNA ‐seq revealed that HDA 5 and HDA 6 co‐regulate gene expression in multiple development processes and pathways.