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Pta RHE 1, a Populus tremula × Populus alba RING ‐H2 protein of the ATL family, has a regulatory role in secondary phloem fibre development
Author(s) -
BaldacciCresp Fabien,
Moussawi Jihad,
Leplé JeanCharles,
Van Acker Rebecca,
Kohler Annegret,
Candiracci Julie,
Twyffels Laure,
Spokevicius Antanas V.,
Bossinger Gerd,
Laurans Françoise,
Brunel Nicole,
Vermeersch Marjorie,
Boerjan Wout,
El Jaziri Mondher,
Baucher Marie
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12867
Subject(s) - phloem , biology , ubiquitin ligase , microbiology and biotechnology , gene , botany , downregulation and upregulation , arabidopsis , ubiquitin , biochemistry , mutant
Summary REALLY INTERESTING NEW GENE ( RING ) proteins play important roles in the regulation of many processes by recognizing target proteins for ubiquitination. Previously, we have shown that the expression of Pta RHE 1 , encoding a Populus tremula × Populus alba RING ‐H2 protein with E3 ubiquitin ligase activity, is associated with tissues undergoing secondary growth. To further elucidate the role of Pta RHE 1 in vascular tissues, we have undertaken a reverse genetic analysis in poplar. Within stem secondary vascular tissues, Pta RHE 1 and its corresponding protein are expressed predominantly in the phloem. The downregulation of Pta RHE 1 in poplar by artificial mi RNA triggers alterations in phloem fibre patterning, characterized by an increased portion of secondary phloem fibres that have a reduced cell wall thickness and a change in lignin composition, with lower levels of syringyl units as compared with wild‐type plants. Following an RNA ‐seq analysis, a biological network involving hormone stress signalling, as well as developmental processes, could be delineated. Several candidate genes possibly associated with the altered phloem fibre phenotype observed in ami RP ta RHE 1 poplar were identified. Altogether, our data suggest a regulatory role for Pta RHE 1 in secondary phloem fibre development.