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The chromatin‐remodeling factor At INO 80 plays crucial roles in genome stability maintenance and in plant development
Author(s) -
Zhang Chi,
Cao Lin,
Rong Liang,
An Zengxuan,
Zhou Wangbin,
Ma Jinbiao,
Shen WenHui,
Zhu Yan,
Dong Aiwu
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12840
Subject(s) - epigenetics , chromatin , biology , gene , chromatin remodeling , mutant , genetics , arabidopsis , repressor , somatic cell , phenotype , transcription factor , arabidopsis thaliana , homologous recombination , microbiology and biotechnology , dna
Summary INO 80 is a conserved chromatin‐remodeling factor in eukaryotes. While a previous study reported that the Arabidopsis thaliana INO 80 (At INO 80) is required for somatic homologous recombination ( HR ), the role of At INO 80 in plant growth and development remains obscure. Here, we identified and characterized two independent atino80 mutant alleles, atino80‐5 and atino80‐6, which display similar and pleiotropic phenotypes, including smaller plant and organ size, and late flowering. Under standard growth conditions, atino80‐5 showed decreased HR ; however, after genotoxic treatment, HR in the mutant increased, accompanied by more DNA double‐strand breaks and stronger cellular responses. Transcription analysis showed that many developmental and environmental responsive genes are overrepresented in the perturbed genes in atino80‐5 . These genes significantly overlapped with the category of H2A.Z body‐enriched genes. At INO 80 also interacts with H2A.Z, and facilitates the enrichment of H2A.Z at the ends of the key flowering repressor genes FLC and MAF 4/5 . Our characterization of the atino80‐5 and atino80‐6 mutants confirms and extends the previous At INO 80 study, and provides perspectives for linking studies of epigenetic mechanisms involved in plant chromatin stability with plant response to developmental and environmental cues.