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Os NAC 2 encoding a NAC transcription factor that affects plant height through mediating the gibberellic acid pathway in rice
Author(s) -
Chen Xu,
Lu Songchong,
Wang Yaofeng,
Zhang Xuan,
Lv Bo,
Luo Liqiong,
Xi Dandan,
Shen Jiabin,
Ma Hong,
Ming Feng
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12819
Subject(s) - gibberellic acid , transcription factor , microbiology and biotechnology , biology , gene , genetics , botany , germination
Summary Plant height and flowering time are key agronomic traits affecting yield in rice ( Oryza sativa ). In this study, we investigated the functions in rice growth and development of Os NAC 2 , encoding a NAC transcription factor in rice. Transgenic plants that constitutively expressed Os NAC 2 had shorter internodes, shorter spikelets, and were more insensitive to gibberellic acid ( GA 3 ). In addition, the levels of GA s decreased in Os NAC 2 overexpression plants, compared with the wild‐type. Moreover, flowering was delayed for approximately 5 days in transgenic lines. The transcription of Hd3a , a flowering‐time related gene, was suppressed in transgenic lines. In addition, transgenic Arabidopsis plants expressing Os NAC 2 were also more insensitive to GA 3 . The expression levels of GA biosynthetic genes Os KO 2 and Os KAO were repressed. The expression of Os SLRL , encoding a repressor in the GA signal pathway, and Os EATB , which encodes a repressor of GA biosynthesis, were both enhanced. Western blotting indicated that DELLA also accumulated at the protein level. Dual‐luciferase reporter analyses, yeast one‐hybrid assays and Ch IP ‐ qPCR suggested that Os NAC 2 directly interacted with the promoter of Os EATB and Os KO 2 . Taken together, we proposed that Os NAC 2 is a negative regulator of the plant height and flowering time, which acts by directly regulating key genes of the GA pathway in rice.