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Identification and characterization of an ABA ‐activated MAP kinase cascade in Arabidopsis thaliana
Author(s) -
Danquah Agyemang,
Zélicourt Axel,
Boudsocq Marie,
Neubauer Jorinde,
Frei dit Frey Nicolas,
Leonhardt Nathalie,
Pateyron Stephanie,
Gwinner Frederik,
Tamby JeanPhilippe,
OrtizMasia Dolores,
Marcote Maria J.,
Hirt Heribert,
Colcombet Jean
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12808
Subject(s) - abscisic acid , mapk/erk pathway , arabidopsis , arabidopsis thaliana , microbiology and biotechnology , mapk cascade , kinase , protein kinase a , mutant , biology , phosphorylation , mitogen activated protein kinase , chemistry , biochemistry , gene
Summary Abscisic acid ( ABA ) is a major phytohormone involved in important stress‐related and developmental plant processes. Recent phosphoproteomic analyses revealed a large set of ABA ‐triggered phosphoproteins as putative mitogen‐activated protein kinase ( MAPK ) targets, although the evidence for MAPK s involved in ABA signalling is still scarce. Here, we identified and reconstituted in vivo a complete ABA ‐activated MAPK cascade, composed of the MAP 3Ks MAP 3K17/18, the MAP 2K MKK 3 and the four C group MAPK s MPK 1/2/7/14. In planta , we show that ABA activation of MPK 7 is blocked in mkk3‐1 and map3k17mapk3k18 plants. Coherently, both mutants exhibit hypersensitivity to ABA and altered expression of a set of ABA ‐dependent genes. A genetic analysis further reveals that this MAPK cascade is activated by the PYR / PYL / RCAR ‐Sn RK 2‐ PP 2C ABA core signalling module through protein synthesis of the MAP 3Ks, unveiling an atypical mechanism for MAPK activation in eukaryotes. Our work provides evidence for a role of an ABA ‐induced MAPK pathway in plant stress signalling.