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The RING finger E3 ligase STRF1 is involved in membrane trafficking and modulates salt‐stress response in Arabidopsis thaliana
Author(s) -
Tian Miaomiao,
Lou Lijuan,
Liu Lijing,
Yu Feifei,
Zhao Qingzhen,
Zhang Huawei,
Wu Yaorong,
Tang Sanyuan,
Xia Ran,
Zhu Baoge,
Serino Giovanna,
Xie Qi
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12797
Subject(s) - ubiquitin ligase , microbiology and biotechnology , endosome , arabidopsis thaliana , brefeldin a , reactive oxygen species , ubiquitin , biochemistry , mutant , intracellular , biology , nadph oxidase , chemistry , golgi apparatus , endoplasmic reticulum , gene
Summary Salt stress is a detrimental factor for plant growth and development. The response to salt stress has been shown to involve components in the intracellular trafficking system, as well as components of the ubiquitin‐proteasome system (UPS). In this article, we have identified in Arabidopsis thaliana a little reported ubiquitin ligase involved in salt‐stress response, which we named STRF1 ( S alt T olerance R ING F inger 1). STRF1 is a member of RING‐H2 finger proteins and we demonstrate that it has ubiquitin ligase activity in vitro . We also show that STRF1 localizes mainly at the plasma membrane and at the intracellular endosomes. strf1‐1 loss‐of‐function mutant seedlings exhibit accelerated endocytosis in roots, and have altered expression of several genes involved in the membrane trafficking system. Moreover, protein trafficking inhibitor, brefeldin A (BFA), treatment has increased BFA bodies in strf1‐1 mutant. This mutant also showed increased tolerance to salt, ionic and osmotic stresses, reduced accumulation of reactive oxygen species during salt stress, and increased expression of AtRbohD , which encodes a nicotinamide adenine dinucleotide phosphate (NADPH) oxidase involved in H 2 O 2 production. We conclude that STRF1 is a membrane trafficking‐related ubiquitin ligase, which helps the plant to respond to salt stress by monitoring intracellular membrane trafficking and reactive oxygen species (ROS) production.