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ENO 2 activity is required for the development and reproductive success of plants, and is feedback‐repressed by A t MBP ‐1
Author(s) -
Eremina Marina,
Rozhon Wilfried,
Yang Saiqi,
Poppenberger Brigitte
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12775
Subject(s) - biology , enolase , arabidopsis , microbiology and biotechnology , transcription factor , phenotype , organogenesis , locus (genetics) , biochemistry , genetics , gene , immunohistochemistry , mutant , immunology
Summary Enolases are key glycolytic enzymes that are highly conserved in prokaryotic and eukaryotic organisms, and are among the most abundant cytosolic proteins. In this study we provide evidence that activity of the enolase ENO 2 is essential for the growth and development of plants. We show that Arabidopsis plants with compromised ENO 2 function, which were generated by mutating the LOS 2/ ENO 2 locus, have severe cellular defects, including reduced cell size and defective cell differentiation with restricted lignification. At the tissue and organ level LOS 2/ ENO 2 ‐deficient plants are characterized by the reduced growth of shoots and roots, altered vascular development and defective secondary growth of stems, impaired floral organogenesis and defective male gametophyte function, resulting in embryo lethality as well as delayed senescence. These phenotypes correlate with reduced lignin and increased salicylic acid contents as well as altered fatty acid and soluble sugar composition. In addition to an enolase the LOS 2/ ENO 2 locus encodes the transcription factor A t MBP ‐1, and here we reveal that this bifunctionality serves to maintain the homeostasis of ENO 2 activity. In summary, we show that in plants enolase function is required for the formation of chorismate‐dependent secondary metabolites, and that this activity is feedback‐inhibited by A t MBP ‐1 to enable the normal development and reproductive success of plants.