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The A rabidopsis leucine‐rich repeat receptor‐like kinase MUSTACHES enforces stomatal bilateral symmetry in A rabidopsis
Author(s) -
Keerthisinghe Sandra,
Nadeau Jeannette A.,
Lucas Jessica R.,
Nakagawa Tsuyoshi,
Sack Fred D.
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12757
Subject(s) - guard cell , cytokinesis , microbiology and biotechnology , microtubule , cytoskeleton , mutant , biology , biophysics , chemistry , cell division , cell , genetics , gene
Summary Stomata display a mirror‐like symmetry that is adaptive for shoot/atmosphere gas exchange. This symmetry includes the facing guard cells around a lens‐shaped and bilaterally symmetric pore, as well as radially arranged microtubule arrays that primarily originate at the pore and then grow outwards. Mutations in MUSTACHES ( MUS ), which encodes a leucine‐rich repeat receptor‐like kinase, disrupt this symmetry, resulting in defects ranging from skewed pores and abnormally focused and depolarized radial microtubule arrays, to paired guard cells that face away from each other, or a severe loss of stomatal shape. Translational MUS pro MUS :triple GFP fusions are expressed in cell plates in most cells types in roots and shoots, and cytokinesis and cell plates are mostly normal in mus mutants. However, in guard mother cells, which divide and then form stomata, MUS expression is notably absent from new cell plates, and instead is peripherally located. These results are consistent with a role for MUS in enforcing wall building and cytoskeletal polarity at the centre of the developing stoma via signalling from the vicinity of the guard cell membrane.