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Seed dormancy cycling in A rabidopsis: chromatin remodelling and regulation of DOG 1 in response to seasonal environmental signals
Author(s) -
Footitt Steven,
Müller Kerstin,
Kermode Allison R.,
FinchSavage William E.
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12735
Subject(s) - cycling , dormancy , microbiology and biotechnology , chemistry , biology , botany , germination , geography , archaeology
Summary The involvement of chromatin remodelling in dormancy cycling in the soil seed bank ( SSB ) is poorly understood. Natural variation between the winter and summer annual A rabidopsis ecotypes C vi and B ur was exploited to investigate the expression of genes involved in chromatin remodelling via histone 2 B ( H 2 B ) ubiquitination/de‐ubiquitination and histone acetylation/deacetylation, the repressive histone methyl transferases CURLY LEAF ( CLF ) and SWINGER ( SWN ), and the gene silencing repressor ROS 1 ( REPRESSOR OF SILENCING 1) and promoter of silencing KYP / SUVH 4 ( KRYPTONITE ), during dormancy cycling in the SSB . ROS 1 expression was positively correlated with dormancy while the reverse was observed for CLF and KYP / SUVH 4 . We propose ROS 1 dependent repression of silencing and a sequential requirement of CLF and KYP / SUVH 4 dependent gene repression and silencing for the maintenance and suppression of dormancy during dormancy cycling. Seasonal expression of H 2 B modifying genes was correlated negatively with temperature and positively with DOG 1 expression, as were histone acetyltransferase genes, with histone deacetylases positively correlated with temperature. Changes in the histone marks H 3 K 4me3 and H 3 K 27me3 were seen on DOG 1 ( DELAY OF GERMINATION 1 ) in C vi during dormancy cycling. H 3 K 4me3 activating marks remained stable along DOG 1 . During relief of dormancy, H 3 K 27me3 repressive marks slowly accumulated and accelerated on exposure to light completing dormancy loss. We propose that these marks on DOG 1 serve as a thermal sensing mechanism during dormancy cycling in preparation for light repression of dormancy. Overall, chromatin remodelling plays a vital role in temporal sensing through regulation of gene expression.