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Arabidopsis DOK 1 encodes a functional dolichol kinase involved in reproduction
Author(s) -
Kanehara Kazue,
Cho Yueh,
Lin YingChen,
Chen ChiaEn,
Yu ChaoYuan,
Nakamura Yuki
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12727
Subject(s) - dolichol , arabidopsis , biology , mutant , biochemistry , saccharomyces cerevisiae , gene , biosynthesis
Summary Dolichol phosphate (Dol‐P) serves as a carrier of complex polysaccharides during protein glycosylation. Dol‐P is synthesized by the phosphorylation of dolichol or the monodephosphorylation of dolichol pyrophosphate (Dol‐ PP ); however, the enzymes that catalyze these reactions remain unidentified in Arabidopsis thaliana . We performed a genome‐wide search for cytidylyltransferase motif‐containing proteins in Arabidopsis, and found that At3g45040 encodes a protein homologous with Sec59p, a dolichol kinase ( DOK ) in Saccharomyces cerevisiae . At3g45040, designated At DOK 1 , complemented defects in the growth and N ‐linked glycosylation of the S. cerevisiae sec59 mutant, suggesting that At DOK 1 encodes a functional DOK . To characterize the physiological roles of At DOK 1 in planta , we isolated two independent lines of T‐ DNA ‐tagged At DOK 1 mutants, dok1 ‐ 1 and dok1 ‐ 2 . The heterozygous plants showed developmental defects in male and female gametophytes, including an aberrant pollen structure, low pollen viability, and short siliques. Additionally, the mutations had incomplete penetrance. These results suggest that At DOK 1 is a functional DOK required for reproductive processes in Arabidopsis.