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Os LOL 1, a C 2 C 2‐type zinc finger protein, interacts with O sb ZIP 58 to promote seed germination through the modulation of gibberellin biosynthesis in Oryza sativa
Author(s) -
Wu Jiahe,
Zhu Chuanfeng,
Pang Jinhuan,
Zhang Xiangrong,
Yang Chunlin,
Xia Guixian,
Tian Yingchuan,
He Chaozu
Publication year - 2014
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12714
Subject(s) - gibberellin , biology , aleurone , complementation , reporter gene , oryza sativa , germination , zinc finger , gene expression , gene , microbiology and biotechnology , biochemistry , botany , transcription factor , mutant
Summary Seed germination is a key developmental process in the plant life cycle that is influenced by various environmental cues and phytohormones through gene expression and a series of metabolism pathways. In the present study, we investigated a C 2 C 2‐type finger protein, Os LOL 1, which promotes gibberellin ( GA ) biosynthesis and affects seed germination in Oryza sativa (rice). We used Os LOL 1 antisense and sense transgenic lines to explore Os LOL 1 functions. Seed germination timing in antisense plants was restored to wild type when exogenous GA 3 was applied. The reduced expression of the GA biosynthesis gene Os KO 2 and the accumulation of ent ‐kaurene were observed during germination in antisense plants. Based on yeast two‐hybrid and firefly luciferase complementation analyses, Os LOL 1 interacted with the basic leucine zipper protein Osb ZIP 58. The results from electrophoretic mobility shift and dual‐luciferase reporter assays showed that Osb ZIP 58 binds the G‐box cis ‐element of the Os KO 2 promoter and activates LUC reporter gene expression, and that interaction between Os LOL 1 and Osb ZIP 58 activates Os KO 2 gene expression. In addition, Os LOL 1 decreased SOD 1 gene expression and accelerated programmed cell death ( PCD ) in the aleurone layer of rice grains. These findings demonstrate that the interaction between OsLOL1 and OsbZIP58 influences GA biosynthesis through the activation of Os KO 2 via Osb ZIP 58, thereby stimulating aleurone PCD and seed germination.