Premium
The V at locus encodes for a CC ‐ NBS ‐ LRR protein that confers resistance to A phis gossypii infestation and A . gossypii ‐mediated virus resistance
Author(s) -
Dogimont Catherine,
Chovelon Veronique,
Pauquet Jerome,
Boualem Adnane,
Bendahmane Abdelhafid
Publication year - 2014
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12690
Subject(s) - aphis gossypii , biology , aphid , cucumis , transgene , virology , gene , locus (genetics) , genetics , botany , aphididae , pest analysis , homoptera
Summary A phis gossypii is a polyphagous sucking aphid and a vector for many viruses. In C ucumis melo , a dominant locus, Vat , confers a high level of resistance to A phis gossypii infestation and to viruses transmitted by this vector. To investigate the mechanism underlying this double resistance, we first genetically dissected the Vat locus. We delimited the double resistance to a single gene that encodes for a coiled‐coil–nucleotide‐binding‐site–leucine‐rich repeat ( CC ‐ NBS ‐ LRR ) protein type. To validate the genetic data, transgenic lines expressing the V at gene were generated and assessed for the double resistance. In this analysis, V at ‐transgenic plants were resistant to A . gossypii infestation as well as A . gossypii ‐mediated virus transmission. When the plants were infected mechanically, virus infection occurred on both transgenic and non‐transgenic control plants. These results confirmed that the cloned CC ‐ NBS ‐ LRR gene mediates both resistance to aphid infestation and virus infection using A . gossypii as a vector. This resistance also invokes a separate recognition and response phases in which the recognition phase involves the interaction of an elicitor molecule from the aphid and V at from the plant. The response phase is not specific and blocks both aphid infestation and virus infection. Sequence analysis of V at alleles suggests a major role of an unusual conserved LRR repeat in the recognition of A . gossypii .