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XTH 20 and XTH 19 regulated by ANAC 071 under auxin flow are involved in cell proliferation in incised Arabidopsis inflorescence stems
Author(s) -
Pitaksaringkarn Weerasak,
Matsuoka Keita,
Asahina Masashi,
Miura Kenji,
SageOno Kimiyo,
Ono Michiyuki,
Yokoyama Ryusuke,
Nishitani Kazuhiko,
Ishii Tadashi,
Iwai Hiroaki,
Satoh Shinobu
Publication year - 2014
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12654
Subject(s) - pith , auxin , microbiology and biotechnology , arabidopsis , transcription factor , cell growth , downregulation and upregulation , chemistry , biology , botany , mutant , biochemistry , gene
Summary One week after partial incision of Arabidopsis inflorescence stems, the repair process in damaged tissue includes pith cell proliferation. Auxin is a key factor driving this process, and ANAC 071 , a transcription factor gene, is upregulated in the distal region of the incised stem. Here we show that XTH 20 and the closely related XTH 19 , members of xyloglucan endotransglucosylase/hydrolases family catalyzing molecular grafting and/or hydrolysis of cell wall xyloglucans, were also upregulated in the distal part of the incised stem, similar to ANAC 071 . XTH 19 was expressed in the proximal incision region after 3 days or after auxin application to the decapitated stem. Horizontal positioning of the plant with the incised side up resulted in decreased Pro DR5 : GUS , ANAC 071 , XTH 20 , and XTH 19 expression and reduced pith cell proliferation. In incised stems of Pro 35S : ANAC 071‐ SRDX plants, expression of XTH 20 and XTH 19 was substantially and moderately decreased, respectively. XTH 20 and XTH 19 expression and pith cell proliferation were suppressed in anac071 plants and were increased in Pro 35S : ANAC 071 plants. Pith cell proliferation was also inhibited in the xth20xth19 double mutant. Furthermore, ANAC 071 bound to the XTH 20 and XTH 19 promoters to induce their expression. This study revealed XTH 20 and XTH 19 induction by auxin via ANAC 071 in the distal part of an incised stem and their involvement in cell proliferation in the tissue reunion process.