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The gibberellin biosynthetic genes At KAO 1 and At KAO 2 have overlapping roles throughout A rabidopsis development
Author(s) -
Regnault Thomas,
Davière JeanMichel,
Heintz Dimitri,
Lange Theo,
Achard Patrick
Publication year - 2014
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12648
Subject(s) - gibberellin , mutant , gene , arabidopsis , biology , subfamily , arabidopsis thaliana , cytochrome p450 , phenotype , complementation , wild type , biochemistry , monooxygenase , abscisic acid , oxidase test , enzyme , genetics
Summary Ent ‐kaurenoic acid oxidase ( KAO ), a class of cytochrome P450 monooxygenases of the subfamily CYP 88A, catalyzes the conversion of ent ‐kaurenoic acid ( KA ) to gibberellin ( GA ) GA 12 , the precursor of all GA s, thereby playing an important role in determining GA concentration in plants. Past work has demonstrated the importance of KAO activity for growth in various plant species. In Arabidopsis , this enzyme is encoded by two genes designated KAO 1 and KAO 2 . In this study, we used various approaches to determine the physiological roles of KAO 1 and KAO 2 throughout plant development. Analysis of gene expression pattern reveals that both genes are mainly expressed in germinating seeds and young developing organs, thus suggesting functional redundancy. Consistent with this, kao1 and kao2 single mutants are indistinguishable from wild‐type plants. By contrast, the kao1 kao2 double mutant exhibits typical non‐germinating GA ‐dwarf phenotypes, similar to those observed in the severely GA ‐deficient ga1‐3 mutant. Phenotypic characterization and quantitative analysis of endogenous GA contents of single and double kao mutants further confirm an overlapping role of KAO 1 and KAO 2 throughout Arabidopsis development.