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The A rabidopsis L ec RK ‐ VI .2 associates with the pattern‐recognition receptor FLS 2 and primes N icotiana benthamiana pattern‐triggered immunity
Author(s) -
Huang PinYao,
Yeh YuHung,
Liu AnChi,
Cheng ChiuPing,
Zimmerli Laurent
Publication year - 2014
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12557
Subject(s) - nicotiana benthamiana , arabidopsis , biology , pattern recognition receptor , arabidopsis thaliana , callose , bimolecular fluorescence complementation , microbiology and biotechnology , ectopic expression , plant disease resistance , innate immune system , genetics , receptor , gene , mutant
Summary Pattern‐triggered immunity ( PTI ) is broad spectrum and manipulation of PTI is believed to represent an attractive way to engineer plants with broad‐spectrum disease resistance. PTI is activated upon perception of microbe‐associated molecular patterns ( MAMP s) by pattern‐recognition receptors ( PRR s). We have recently demonstrated that the L‐type lectin receptor kinase‐ VI .2 (Lec RK ‐ VI .2) positively regulates Arabidopsis thaliana PTI . Here we show through in vitro pull‐down, bimolecular fluorescence complementation and co‐immunoprecipitation analyses that Lec RK ‐ VI .2 associates with the PRR FLS 2. We also demonstrated that Lec RK ‐ VI .2 from the cruciferous plant Arabidopsis remains functional after interfamily transfer to the Solanaceous plant Nicotiana benthamiana . Wild tobacco plants ectopically expressing Lec RK ‐ VI .2 were indeed more resistant to virulent hemi‐biotrophic and necrotrophic bacteria, but not to the fungal pathogen Botrytis cinerea suggesting that, as with Arabidopsis, the Lec RK ‐ VI .2 protective effect in N. benthamiana is bacteria specific. Ectopic expression of Lec RK ‐ VI .2 in N. benthamiana primed PTI ‐mediated reactive oxygen species production, mitogen‐activated protein kinase ( MAPK ) activity, callose deposition and gene expression upon treatment with the MAMP flagellin. Our findings identified Lec RK ‐ VI .2 as a member of the FLS 2 receptor complex and suggest that heterologous expression of components of PRR complexes can be used as tools to engineer plant disease resistance to bacteria.